When working with GC-rich DNA regions (e.g. promoters, repetitive elements, gene families, high GC islands), real-time PCR (qPCR) with probes (e.g. hydrolysis / TaqMan probes) can be challenging. Secondary structures,
Continue readingOptimizing Probe-Based qPCR for High GC Content Templates Using Taq Pro HighGC Mix
Introduction Quantitative real-time PCR (qPCR) is a foundational tool in molecular biology and gene expression studies. Two major fluorescence detection strategies coexist in widespread use: (1) intercalating dye systems (commonly
Continue readingComparing SYBR qPCR Master Mix with Probe-Based Systems: Strengths and Limitations
Introduction In experimental models of genotoxic stress, one common strategy is to induce DNA lesions (via ROS, oxidants, ionizing radiation, etc.), and then monitor repair kinetics or residual damage. Many
Why Platelet Diluting Fluids Are Essential Platelet counts are one of the most fundamental laboratory measurements in hematology. They provide insights into thrombocytopenia, thrombocytosis, and clotting abnormalities, and they remain
Continue readingComparing Rees-Ecker Fluid with Other Platelet Diluting Fluids
Introduction: Why Quality Control Cannot Be an Afterthought High-throughput respiratory PCR panels are central tools for detecting pathogens such as influenza viruses, respiratory syncytial virus (RSV), parainfluenza, adenoviruses, and coronaviruses.
Continue readingIntegrating Quality Control into High-Throughput Respiratory PCR Panel Workflows
The Central Role of AAV in Research and Bioproduction Recombinant adeno-associated virus (rAAV) is now one of the most widely used viral vector systems. It is applied in neuroscience, immunology,
Continue readingComparing PEI-Mediated Transfection with Alternative Methods for AAV Production
Introduction Avian influenza virus (AIV) is one of the most economically significant pathogens in poultry production. Fast, reliable detection is essential for outbreak control, flock management, and trade monitoring. While
Continue readingPrinciples of AIV Antigen Rapid Tests in Poultry Diagnostics
Introduction In high-throughput next-generation sequencing (NGS), the choice of indexing primers is one of the most critical steps for achieving accurate, reproducible, and contamination-free results. Among the available indexing solutions,
Continue readingPractical Considerations When Using Stubby UDI Primers in Library Preparation
Introduction Leishman stain is one of the most widely used Romanowsky-type stains in laboratories around the world. It is a critical reagent for hematology, parasitology, and cell morphology studies, providing
Introduction The polymerase chain reaction (PCR) is one of the most widely used techniques in molecular biology, enabling the amplification of DNA sequences for applications ranging from cloning to sequencing
Continue readingRole of dUTP in DNA Polymerase Reactions and PCR Optimization