Understanding ZDHHC9 Antibody: Functions, Applications, and Research

What is ZDHHC9?

ZDHHC9 (Zinc Finger DHHC-Type Palmitoyltransferase 9) is an enzyme involved in protein palmitoylation, a post-translational modification crucial for protein stability and membrane localization. This gene is particularly significant in neurological development and cancer research (NCBI).

Role of ZDHHC9 in Cellular Function

ZDHHC9 plays a role in modulating Ras signaling pathways, which are essential for cell growth, differentiation, and apoptosis (National Cancer Institute). Mutations in ZDHHC9 have been linked to X-linked intellectual disabilities (NIH Genetic Home Reference).

ZDHHC9 and Disease Associations

1. Neurological Disorders – Research has shown that mutations in ZDHHC9 contribute to intellectual disability and epilepsy (National Institute of Neurological Disorders and Stroke).
2. Cancer Research – ZDHHC9 has been linked to oncogenic pathways, influencing tumor progression (National Cancer Institute).
3. Infectious Diseases – Studies suggest that palmitoylation by ZDHHC9 may influence viral pathogenesis (CDC).
4. Cardiovascular Implications – Some studies suggest that ZDHHC9 may play a role in vascular function and cardiovascular diseases (American Heart Association).

Applications of ZDHHC9 Antibody

1. Western Blotting

ZDHHC9 antibodies are widely used in Western blot assays to detect protein expression levels (Harvard Medical School).

2. Immunofluorescence & Immunohistochemistry

These antibodies enable researchers to study subcellular localization in brain tissue and cancerous cells (Stanford University).

3. Protein Interaction Studies

By using co-immunoprecipitation, scientists explore protein-protein interactions involving ZDHHC9 (Cold Spring Harbor Laboratory).

4. Flow Cytometry

ZDHHC9 antibodies are also utilized in flow cytometry assays for cell surface and intracellular protein analysis (Johns Hopkins University).

Where to Source ZDHHC9 Antibodies?

Several academic and government institutions provide detailed antibody validation data:

• Antibody Registry (antibodyregistry.org)
• NIH Antibody Database (NIH)
• Protein Atlas (proteinatlas.org)
• Addgene Plasmid Repository (Addgene)

Recent Advances in ZDHHC9 Research

Genomic Studies

Advancements in CRISPR-based genome editing have facilitated targeted studies of ZDHHC9 function (Broad Institute).

Structural Biology Insights

Cryo-EM studies have revealed the 3D structure of ZDHHC9, aiding in drug development (National Center for Biotechnology Information).

Therapeutic Potential

ZDHHC9 inhibitors are being explored as potential treatments for Ras-driven cancers (National Cancer Institute).

Machine Learning in Antibody Research

New computational tools using artificial intelligence are accelerating antibody discovery and binding efficiency predictions (MIT).

Future Directions for ZDHHC9 Research

1. Personalized Medicine – Investigating ZDHHC9 genetic variations for targeted therapies (National Human Genome Research Institute).
2. New Drug Development – Exploring small molecule inhibitors to modulate ZDHHC9 activity (FDA Drug Development).
3. Broader Neurological Studies – Further research into ZDHHC9’s impact on neurodevelopmental disorders (National Institute of Mental Health).

Conclusion

ZDHHC9 is a crucial enzyme implicated in various biological processes, from neurodevelopment to oncogenesis. The availability of high-quality antibodies has accelerated research efforts in biomedicine and drug discovery. For more in-depth resources, visit authoritative sites like NCBI, NIH, and CDC.

References

• National Institutes of Health (NIH)
• National Cancer Institute
• National Center for Biotechnology Information (NCBI)
• Centers for Disease Control and Prevention (CDC)
• Harvard Medical School
• Stanford University
• Cold Spring Harbor Laboratory
• American Heart Association
• Johns Hopkins University
• Broad Institute
• MIT CSAIL
• National Human Genome Research Institute
• FDA Drug Development
• National Institute of Mental Health